DEAE Affi-Gel Blue Gel from Bio-Rad

Product Specs
ItemDEAE Affi-Gel Blue Gel
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1537307
Brand/TrademarkLSG PFTL

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin GAnalytical and Bioanalytical ChemistryMarch 1, 2023Yuye Zhou, Alexander Jönsson, Drago Sticker, Guojun Zhou, Zishuo Yuan, Jörg P. Kutter, Åsa Emmerwas purified in a Millipore Synergy® 185 (Bedford, MA, USA) to a resistivity of 18.2 MΩ·cm at 25 °C. DEAE Affi-Gel® Blue Gel (DEAE) used for IgG extraction was obtained from Bio-RAD, Hercules, USA. Pierce® C18 Tips, 100 µL bed

(2) Generation of Amyloid-β Is Reduced by the Interaction of Calreticulin with Amyloid Precursor Protein, Presenilin and NicastrinPLoS ONEApril 9, 2013Nina Stemmer, Elena Strekalova, Nevena Djogo, Frank Plöger, Gabriele Loers, David Lutz, Friedrich Buck, Marek Michalak, Melitta Schachner, Ralf Kleenesolution was used to immunize rabbits (2 boosts, 500 µl per boost). For isolation of the IgG fractions the DEAE Affi-Gel Blue Gel kit (Bio-Rad, München, Germany) was used according to the manufacturer's instructions. Briefly, DEAE Affi-Gel Blue gel

(3) Serum albumin alters the expression of iron-controlled genes in Pseudomonas aeruginosaMicrobiology (Reading)February 1, 2012Cassandra Kruczek, Mitchell Wachtel, Magdy S. Alabady, Paxton R. Payton, Jane A. Colmer-Hamood, Abdul N. HamoodAHS into different fractions (¢3/,3 kDa and ¢50/,50 kDa). Albumin was absorbed from ABS or AHS using DEAE Affi-Gel Blue gel columns (Bio-Rad) according to the manufacturer’s recommendations. To determine effects of protein degradation,

(4) Formate hydrogenlyase in the hyperthermophilic archaeon, Thermococcus litoralisBMC MicrobiologyJune 3, 2008Mária Takács, András Tóth, Balázs Bogos, András Varga, Gábor Rákhely, Kornél L Kovácswere carried out according to standard methods [33]. The crude IgG fraction was further purified on DEAE Affi-Gel Blue Gel column (Bio-Rad). Preimmune serum was also prepared and used in control experiments to prove the specificity

(5) DYC-1, a Protein Functionally Linked to Dystrophin inCaenorhabditis elegansIs Associated with the Dense Body, Where It Interacts with the Muscle LIM Domain Protein ZYX-1Molecular Biology of the CellMarch 1, 2008Claire Lecroisey, Edwige Martin, Marie-Christine Mariol, Laure Granger, Yannick Schwab, Michel Labouesse, Laurent Ségalat, Kathrin Gieselerethanolamine, pH 8; and 10 mM Tris, pH 7.5. Five milliliters of the rabbit antiserum were prepared on DEAE Affi-Gel Blue Gel (Bio-Rad) following the manufacturer’s directions. This prepared serum was precleared of anti-GST antibodies

(6) Retinoid regulated association of transcriptional co-regulators and the polycomb group protein SUZ12 with the retinoic acid response elements of Hoxa1, RARbeta(2), and Cyp26A1 in F9 embryonal carcinoma cellsJournal of Molecular BiologySeptember 14, 2007Robert F. Gillespie, Lorraine J. Gudascarboxy terminal of RARγ. Polyclonal anti-RARγ IgG was purified from the crude serum through use of a DEAE Affi-gel Blue Gel column (BioRad, Hercules, CA). Anti-RXRα (D-20, sc-553), anti-pCIP (M-397, sc-9119), anti-p300 (N-15, sc-584)

(7) Trypanosoma cruzi: Molecular characterization of an RNA binding protein differentially expressed in the parasite life cycleExperimental ParasitologySeptember 1, 2007Leticia PÃ©rez-DÃaz, MarÃa Ana Duhagon, Pablo Smircich, JosÃ© Sotelo-Silveira, Carlos Robello, Marco Aurelio Krieger, Samuel Goldenberg, Noreen Williams, Bruno Dallagiovanna, Beatriz Garateach time) and serum was obtained two weeks after the last boost. The polyclonal serum was purified on DEAE Affi-Gel®Blue Gel columns (BioRad) following manufacturer's instructions. Afterwards affinity purification using GSTTcRBP19

(8) The Extracellular Membrane-Proximal Domain of Human Membrane IgE Controls Apoptotic Signaling of the B Cell Receptor in the Mature B Cell Line A20The Journal of ImmunologySeptember 15, 2006Monica Poggianella, Marco Bestagno, Oscar R. Burronepurification and IgG Fab production Sera, obtained from vaccinated guinea pigs, were purified using DEAE Affi-Gel BLUE GEL (Bio-Rad), following the manufacturer’s protocol. IgG fractions were quantified by SDS-PAGE, followed

(9) Autoantigen, innate immunity, and T cells cooperate to break B cell tolerance during bacterial infectionJournal of Clinical InvestigationAugust 1, 2005Pauline Soulas, Anne Woods, Benoit Jaulhac, Anne-Marie Knapp, Jean-Louis Pasquali, Thierry Martin, Anne-Sophie KorganowInc.) were cleared by centrifugation and decomplemented. Each pool of serum was then loaded onto a DEAE Affi-Gel Blue Gel column (Bio-Rad Laboratories) according to the manufacturer’s instructions. Eluted fractions were concentrated

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